The present study include a simple, sensitive and specific RPHPLC method development and validation for the Doxophylline in tablet dosage form. The λmax found to be 276 nm by taking Acetonitrile and Buffer (20:80) as solvent.The validation of the proposed method was carried out as per ICH Guidelines. It was found that the drug was shown the linearity between the ranges 60-160%. The regression of curve was y = 12817x + 123218, r2 =0.9996. The developed method was found to be with % RSD 0.026. The % RSD for intra-day and inter-day precision was lower than 2%. The percentage recovery values of pure drug were in between 90-110%. The ruggedness of the method was studied by taking in account of different analyst and by varying the temperature. Based on the performance characteristic the proposed RPHPLC method was found to suitable for the estimation of Doxophylline in bulk and pharmaceutical dosage forms.
Loading....